Isolation of 91 polymorphic microsatellite loci in the western Mediterranean endemic Carex helodes (Cyperaceae)

Author

Juan Arroyo, Marcial Escudero, Pedro Jordano

Published

January 1, 2016

Doi

10.3732/apps.1500085

Juan Arroyo, Marcial Escudero, Pedro Jordano 2016. Isolation of 91 polymorphic microsatellite loci in the western Mediterranean endemic Carex helodes (Cyperaceae). Applications in Plant Sciences 4. https://doi.org/10.3732/apps.1500085

Publication Details

Published in: Applications in Plant Sciences  Open Access: Yes

BibTeX

@article{RN57993,
   author = {Arroyo, J. M. and Escudero, M. and Jordano, P.},
   title = {Isolation of 91 polymorphic microsatellite loci in the Western Mediterranean endemic Carex Helodes (Cyperaceae)},
   journal = {Applications in Plant Sciences},
   volume = {4},
   number = {1},
   note = {Dp6rh
Times Cited:0
Cited References Count:13},
   abstract = {Premise of the study: Microsatellite primers were developed for Carex helodes (Cyperaceae), a western Mediterranean endemic that is locally distributed in southern Portugal and southwestern Spain and rare in northern Morocco.
Methods and Results: One hundred nine nuclear microsatellite markers were developed using a shotgun pyrosequencing method, resulting in 91 polymorphic and 18 monomorphic loci when tested using 19 individuals sampled from five populations from Portugal, Spain, and Morocco. Loci averaged 3.23 alleles per locus (SD = 1.15). In a single population (Cortelha population, Portugal), the 34 most polymorphic loci showed a mean observed heterozygosity of 0.357 (SD = 0.292) and mean expected heterozygosity of 0.384 (SD = 0.255).
Conclusions: Next-generation sequencing allowed us to develop a high number of genetic markers with levels of polymorphism adequate to study gene flow among populations. However, when genotyping the individuals within a population, we found low levels of variation.},
   keywords = {carex helodes
cyperaceae
endemism
sedge
shotgun sequencing
simple sequence repeat (ssr) marker
population-genetics
software
windows
linux},
   ISSN = {2168-0450},
   DOI = {ARTN 1500085
10.3732/apps.1500085},
   url = {<Go to ISI>://WOS:000378625500004
https://onlinelibrary.wiley.com/doi/pdf/10.3732/apps.1500085},
   year = {2016},
   type = {Journal Article}
}