Isolation and characterization of 20 microsatellite loci for laurel species (Laurus, Lauraceae)

Author

Juan Arroyo, Cristina Rigueiro, Rocío Rodríguez‐Arcos et al.

Published

April 1, 2010

Doi

10.3732/ajb.1000069

Juan Arroyo, Cristina Rigueiro, Rocío Rodríguez‐Arcos et al 2010. Isolation and characterization of 20 microsatellite loci for laurel species (Laurus, Lauraceae). American Journal of Botany 97: E26–E30. https://doi.org/10.3732/ajb.1000069

Publication Details

Published in: American Journal of Botany  Open Access: Yes

BibTeX

@article{RN57986,
   author = {Arroyo, J. M. and Rigueiro, C. and Rodriguez, R. and Hampe, A. and Valido, A. and Rodriguez-Sanchez, F. and Jordano, P.},
   title = {Isolation and characterization of 20 microsatellite loci for laurel species (Laurus, Lauraceae)},
   journal = {American Journal of Botany},
   volume = {97},
   number = {5},
   pages = {E26–E30},
   note = {589uo
Times Cited:6
Cited References Count:10},
   abstract = {Premise of the study: Microsatellite primers were developed for the evergreen tree Laurus to investigate population genetic structure and patterns of gene flow via animal-dispersed pollen and seeds.
Methods and Results: Twenty polymorphic nuclear microsatellite markers were developed using CA, GA, AAC, and ATG n-enriched genomic libraries. Given the tetraploidy of the sampled populations, we analyzed our data both as dominant loci and as codominant genotypic data to calculate allele frequencies and genetic diversity. A total of 196 and 222 alleles were found in 37 Mediterranean (L. nobilis) and 26 Macaronesian islands (L. azorica) individuals, respectively.
Conclusions: Levels of polymorphism of the reported markers are adequate for studies of diversity and parentage in natural populations of this Tertiary relict tree.},
   keywords = {genetic diversity
laurus azorica
laurus nobilis
polyploidy
ssr},
   ISSN = {0002-9122},
   DOI = {10.3732/ajb.1000069},
   url = {<Go to ISI>://WOS:000277179900020},
   year = {2010},
   type = {Journal Article}
}